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NMR Determination of Protein pK(a) Values in the Solid State

Charged residues play an important role in defining key mechanistic features in many biomolecules. Determining the pK(a) values of large, membrane or fibrillar proteins can be challenging with traditional methods. In this study we show how solid-state NMR is used to monitor chemical shift changes du...

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Autores principales: Schmidt, Heather L. Frericks, Shah, Gautam J., Sperling, Lindsay J., Rienstra, Chad M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885713/
https://ncbi.nlm.nih.gov/pubmed/20563223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jz1004413
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