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NMR Determination of Protein pK(a) Values in the Solid State

Charged residues play an important role in defining key mechanistic features in many biomolecules. Determining the pK(a) values of large, membrane or fibrillar proteins can be challenging with traditional methods. In this study we show how solid-state NMR is used to monitor chemical shift changes du...

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Bibliografiske detaljer
Main Authors: Schmidt, Heather L. Frericks, Shah, Gautam J., Sperling, Lindsay J., Rienstra, Chad M.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885713/
https://ncbi.nlm.nih.gov/pubmed/20563223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jz1004413
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