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NMR Determination of Protein pK(a) Values in the Solid State

Charged residues play an important role in defining key mechanistic features in many biomolecules. Determining the pK(a) values of large, membrane or fibrillar proteins can be challenging with traditional methods. In this study we show how solid-state NMR is used to monitor chemical shift changes du...

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Autors principals: Schmidt, Heather L. Frericks, Shah, Gautam J., Sperling, Lindsay J., Rienstra, Chad M.
Format: Artigo
Idioma:Inglês
Publicat: 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885713/
https://ncbi.nlm.nih.gov/pubmed/20563223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jz1004413
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