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NMR determination of pK(a) values in α-synuclein

The intrinsically unfolded protein α-synuclein has an N-terminal domain with seven imperfect KTKEGV sequence repeats and a C-terminal domain with a large proportion of acidic residues. We characterized pK(a) values for all 26 sites in the protein that ionize below pH 7 using 2D (1)H-(15)N HSQC and 3...

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Detalhes bibliográficos
Main Authors: Croke, Robyn L, Patil, Sharadrao M, Quevreaux, Jason, Kendall, Debra A, Alexandrescu, Andrei T
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3048411/
https://ncbi.nlm.nih.gov/pubmed/21280118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.556
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