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High-yield expression and purification of isotopically labeled cytochrome P450 monooxygenases for solid-state NMR spectroscopy

Cytochrome P450 monooxygenases (P450s), which represent the major group of drug metabolizing enzymes in humans, also catalyze important synthetic and detoxicative reactions in insects, plants and many microbes. Flexibilities in their catalytic sites and membrane associations are thought to play cent...

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Main Authors: Rupasinghe, Sanjeewa G., Duan, Hui, Frericks Schmidt, Heather L., Berthold, Deborah A., Rienstra, Chad M., Schuler, Mary A.
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2192630/
https://ncbi.nlm.nih.gov/pubmed/18005930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2007.09.009
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