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A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector
BACKGROUND: Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of proliferation state. Furthermor...
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| Опубликовано в: : | BMC Biotechnol |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5331646/ https://ncbi.nlm.nih.gov/pubmed/28245848 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-017-0341-x |
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