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In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP

Recently, a number of study have shown the ligand-dependent allosteric ribozymes can be harnessed as biosensors, high-throughput screening, and agents for the control of gene expression in vivo, called artificial riboswitches. In this chapter, we describe how in vitro selection can be used to create...

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Publicat a:Methods Mol Biol
Autors principals: Furukawa, Kazuhiro, Gu, Hongzhou, Breaker, Ronald R.
Format: Artigo
Idioma:Inglês
Publicat: 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5325115/
https://ncbi.nlm.nih.gov/pubmed/24549622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-755-6_15
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