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In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP
Recently, a number of study have shown the ligand-dependent allosteric ribozymes can be harnessed as biosensors, high-throughput screening, and agents for the control of gene expression in vivo, called artificial riboswitches. In this chapter, we describe how in vitro selection can be used to create...
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| Publié dans: | Methods Mol Biol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2014
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5325115/ https://ncbi.nlm.nih.gov/pubmed/24549622 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-755-6_15 |
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