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One-step random mutagenesis by error-prone rolling circle amplification
In vitro random mutagenesis is a powerful tool for altering properties of enzymes. We describe here a novel random mutagenesis method using rolling circle amplification, named error-prone RCA. This method consists of only one DNA amplification step followed by transformation of the host strain, with...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2004
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC528823/ https://ncbi.nlm.nih.gov/pubmed/15507684 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh147 |
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