One-step random mutagenesis by error-prone rolling circle amplification
In vitro random mutagenesis is a powerful tool for altering properties of enzymes. We describe here a novel random mutagenesis method using rolling circle amplification, named error-prone RCA. This method consists of only one DNA amplification step followed by transformation of the host strain, with...
Na minha lista:
| Udgivet i: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2004
|
| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC528823/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15507684/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh147 |
| Tags: |
Ingen Tags, Vær først til at tagge denne postø!
|