One-step random mutagenesis by error-prone rolling circle amplification
In vitro random mutagenesis is a powerful tool for altering properties of enzymes. We describe here a novel random mutagenesis method using rolling circle amplification, named error-prone RCA. This method consists of only one DNA amplification step followed by transformation of the host strain, with...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC528823/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15507684/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh147 |
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