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Micelle-enhanced bioorthogonal labeling of genetically-encoded azido groups on the lipid-embedded surface of a GPCR
Genetically-encoded p-azido-phenylalanine (azF) residues in G protein-coupled receptors (GPCRs) can be targeted with dibenzocyclooctyne (DIBO) modified fluorescent probes using strain-promoted [3+2] azide–alkyne cycloaddition (SpAAC). Here we show that azF residues situated on the transmembrane surf...
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| Udgivet i: | Chembiochem |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5287413/ https://ncbi.nlm.nih.gov/pubmed/25962668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201500030 |
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