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FTIR analysis of GPCR activation using azido probes
We demonstrate the site-directed incorporation of an IR-active amino acid, p-azido-l-phenylalanine (azidoF, 1), into the G protein–coupled receptor rhodopsin using amber codon suppression technology. The antisymmetric stretch vibration of the azido group absorbs at ~2,100 cm(−1) in a clear spectral...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2875874/ https://ncbi.nlm.nih.gov/pubmed/19396177 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.167 |
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