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Mapping the Ligand-binding Site on a GPCR Using Genetically-encoded Photocrosslinkers

We developed a general cell-based photocrosslinking approach to investigate the binding interfaces necessary for the formation of G protein-coupled receptor (GPCR) signaling complexes. The two photoactivatable unnatural amino acids p-benzoyl-L-phenylalanine and p-azido-L-phenylalanine were incorpora...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Grunbeck, Amy, Huber, Thomas, Sachdev, Pallavi, Sakmar, Thomas P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3099303/
https://ncbi.nlm.nih.gov/pubmed/21417335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi200214r
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