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Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.

A simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features of Tn10 and Tn5; (ii) the resistances to the herbicide bialaphos, to mercuric salts and organomercur...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Herrero, M, de Lorenzo, V, Timmis, K N
Format: Artigo
Langue:Inglês
Publié: 1990
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC526845/
https://ncbi.nlm.nih.gov/pubmed/2172216
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