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Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.

A simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features of Tn10 and Tn5; (ii) the resistances to the herbicide bialaphos, to mercuric salts and organomercur...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Main Authors: Herrero, M, de Lorenzo, V, Timmis, K N
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC526845/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2172216/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.11.6557-6567.1990
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