Two-way molecular ligation for efficient conversion of monomeric hepatitis B virus DNA constructs into tandem dimers

Replication of the 3.2-kb hepatitis B virus (HBV) genome is driven by the covalently closed circular (ccc) DNA in the nucleus, from which four classes of co-terminal RNAs are transcribed. Genome replication requires just the 3.5-kb pregenomic RNA, which is terminally redundant. Cloning the full-leng...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Zong, Li, Qin, Yanli, Jia, Haodi, Zhou, Huailiang, Chen, Chaoyang, Qiao, Ke, Zhang, Jiming, Wang, Yongxiang, Li, Jisu, Tong, Shuping
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5257262/
https://ncbi.nlm.nih.gov/pubmed/27025357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2016.03.012
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