A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library.

A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step, the portions of partial clones that extend out from the reg...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Gibbons, I R, Asai, D J, Ching, N S, Dolecki, G J, Mocz, G, Phillipson, C A, Ren, H, Tang, W J, Gibbons, B H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52549/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1833761/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.19.8563
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