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A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library.

A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step, the portions of partial clones that extend out from the reg...

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Detalhes bibliográficos
Main Authors: Gibbons, I R, Asai, D J, Ching, N S, Dolecki, G J, Mocz, G, Phillipson, C A, Ren, H, Tang, W J, Gibbons, B H
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC52549/
https://ncbi.nlm.nih.gov/pubmed/1833761
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