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Time-resolved neutron scattering provides new insight into protein substrate processing by a AAA+ unfoldase

We present a combination of small-angle neutron scattering, deuterium labelling and contrast variation, temperature activation and fluorescence spectroscopy as a novel approach to obtain time-resolved, structural data individually from macromolecular complexes and their substrates during active bioc...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Ibrahim, Ziad, Martel, Anne, Moulin, Martine, Kim, Henry S., Härtlein, Michael, Franzetti, Bruno, Gabel, Frank
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5244417/
https://ncbi.nlm.nih.gov/pubmed/28102317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep40948
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