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Time-resolved neutron scattering provides new insight into protein substrate processing by a AAA+ unfoldase
We present a combination of small-angle neutron scattering, deuterium labelling and contrast variation, temperature activation and fluorescence spectroscopy as a novel approach to obtain time-resolved, structural data individually from macromolecular complexes and their substrates during active bioc...
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| Published in: | Sci Rep |
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| Main Authors: | , , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Nature Publishing Group
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5244417/ https://ncbi.nlm.nih.gov/pubmed/28102317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep40948 |
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