Massively parallel digital transcriptional profiling of single cells

Characterizing the transcriptome of individual cells is fundamental to understanding complex biological systems. We describe a droplet-based system that enables 3′ mRNA counting of tens of thousands of single cells per sample. Cell encapsulation, of up to 8 samples at a time, takes place in ∼6 min,...

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Veröffentlicht in:Nat Commun
Hauptverfasser: Zheng, Grace X. Y., Terry, Jessica M., Belgrader, Phillip, Ryvkin, Paul, Bent, Zachary W., Wilson, Ryan, Ziraldo, Solongo B., Wheeler, Tobias D., McDermott, Geoff P., Zhu, Junjie, Gregory, Mark T., Shuga, Joe, Montesclaros, Luz, Underwood, Jason G., Masquelier, Donald A., Nishimura, Stefanie Y., Schnall-Levin, Michael, Wyatt, Paul W., Hindson, Christopher M., Bharadwaj, Rajiv, Wong, Alexander, Ness, Kevin D., Beppu, Lan W., Deeg, H. Joachim, McFarland, Christopher, Loeb, Keith R., Valente, William J., Ericson, Nolan G., Stevens, Emily A., Radich, Jerald P., Mikkelsen, Tarjei S., Hindson, Benjamin J., Bielas, Jason H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2017
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5241818/
https://ncbi.nlm.nih.gov/pubmed/28091601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms14049
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