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The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E. coli cells are not fully protected by...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Fomenkov, A, Xiao, J P, Dila, D, Raleigh, E, Xu, S Y
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 1994
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC523701/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8036170/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.12.2399
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