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The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E. coli cells are not fully protected by...

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Main Authors: Fomenkov, A, Xiao, J P, Dila, D, Raleigh, E, Xu, S Y
格式: Artigo
語言:Inglês
出版: 1994
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC523701/
https://ncbi.nlm.nih.gov/pubmed/8036170
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