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The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.
A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E. coli cells are not fully protected by...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1994
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC523701/ https://ncbi.nlm.nih.gov/pubmed/8036170 |
| タグ: |
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