BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism

The GGCC-specific restriction endonuclease BspRI is one of the few Type IIP restriction endonucleases, which were suggested to be a monomer. Amino acid sequence information obtained by Edman sequencing and mass spectrometry analysis was used to clone the gene encoding BspRI. The bspRIR gene is locat...

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Autores principales: Raskó, Tamás, Dér, András, Klement, Éva, Ślaska-Kiss, Krystyna, Pósfai, Eszter, Medzihradszky, Katalin F., Marshak, Daniel R., Roberts, Richard J., Kiss, Antal
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2010
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2978348/
https://ncbi.nlm.nih.gov/pubmed/20587501
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq567
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