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Footprinting RNA-protein complexes following gel retardation assays: application to the R-17-procoat-RNA and tat--TAR interactions.

RNA-protein complexes isolated following a gel retardation assay can be footprinted within the gel matrix using the chemical nuclease activities of 4,7-dimethyl-, 5,6-dimethyl-, and 3,4,7,8-tetramethyl-1,10-phenanthroline-copper. These complexes are more reactive than 1,10-phenanthroline-copper but...

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Detalhes bibliográficos
Main Authors: Pearson, L, Chen, C B, Gaynor, R P, Sigman, D S
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC523682/
https://ncbi.nlm.nih.gov/pubmed/8036153
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