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Footprinting RNA-protein complexes following gel retardation assays: application to the R-17-procoat-RNA and tat--TAR interactions.
RNA-protein complexes isolated following a gel retardation assay can be footprinted within the gel matrix using the chemical nuclease activities of 4,7-dimethyl-, 5,6-dimethyl-, and 3,4,7,8-tetramethyl-1,10-phenanthroline-copper. These complexes are more reactive than 1,10-phenanthroline-copper but...
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Main Authors: | , , , |
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Format: | Artigo |
Jezik: | Inglês |
Izdano: |
1994
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Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC523682/ https://ncbi.nlm.nih.gov/pubmed/8036153 |
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