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Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

The serotonin transporter is a sodium and chloride-coupled transporter that "pumps" extracellular serotonin into cells. S-citalopram is a drug used to treat depression and anxiety by binding to the serotonin transporter with high-affinity, blocking serotonin reuptake. Here we report an eff...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Coleman, Jonathan A., Green, Evan M., Gouaux, Eric
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226304/
https://ncbi.nlm.nih.gov/pubmed/27929454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54792
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