Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Even with the revolution of gene-targeting technologies led by CRISPR-Cas9, genetic modification of human pluripotent stem cells (hPSCs) is still time consuming. Comparative studies that use recombinant lines with transgenes integrated into safe harbor loci could benefit from approaches that use sit...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Ordovás, Laura, Boon, Ruben, Pistoni, Mariaelena, Chen, Yemiao, Sambathkumar, Rangarajan, Helsen, Nicky, Vanhove, Jolien, Berckmans, Pieter, Cai, Qing, Vanuytsel, Kim, Raitano, Susanna, Verfaillie, Catherine M.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Medicine
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226264/
https://ncbi.nlm.nih.gov/pubmed/27911376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54718
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