Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Even with the revolution of gene-targeting technologies led by CRISPR-Cas9, genetic modification of human pluripotent stem cells (hPSCs) is still time consuming. Comparative studies that use recombinant lines with transgenes integrated into safe harbor loci could benefit from approaches that use sit...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Autores principales: Ordovás, Laura, Boon, Ruben, Pistoni, Mariaelena, Chen, Yemiao, Sambathkumar, Rangarajan, Helsen, Nicky, Vanhove, Jolien, Berckmans, Pieter, Cai, Qing, Vanuytsel, Kim, Raitano, Susanna, Verfaillie, Catherine M.
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2016
Materias:
Medicine
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226264/
https://ncbi.nlm.nih.gov/pubmed/27911376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54718
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