Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Even with the revolution of gene-targeting technologies led by CRISPR-Cas9, genetic modification of human pluripotent stem cells (hPSCs) is still time consuming. Comparative studies that use recombinant lines with transgenes integrated into safe harbor loci could benefit from approaches that use sit...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:J Vis Exp
Egile Nagusiak: Ordovás, Laura, Boon, Ruben, Pistoni, Mariaelena, Chen, Yemiao, Sambathkumar, Rangarajan, Helsen, Nicky, Vanhove, Jolien, Berckmans, Pieter, Cai, Qing, Vanuytsel, Kim, Raitano, Susanna, Verfaillie, Catherine M.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: MyJove Corporation 2016
Gaiak:
Medicine
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226264/
https://ncbi.nlm.nih.gov/pubmed/27911376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54718
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