Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Even with the revolution of gene-targeting technologies led by CRISPR-Cas9, genetic modification of human pluripotent stem cells (hPSCs) is still time consuming. Comparative studies that use recombinant lines with transgenes integrated into safe harbor loci could benefit from approaches that use sit...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Ordovás, Laura, Boon, Ruben, Pistoni, Mariaelena, Chen, Yemiao, Sambathkumar, Rangarajan, Helsen, Nicky, Vanhove, Jolien, Berckmans, Pieter, Cai, Qing, Vanuytsel, Kim, Raitano, Susanna, Verfaillie, Catherine M.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2016
Fag:
Medicine
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226264/
https://ncbi.nlm.nih.gov/pubmed/27911376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54718
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!

Lignende værker