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A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
The CRISPR-associated endonuclease Cas9 can edit particular genomic loci directed by a single guide RNA (gRNA). The CRISPR/Cas9 system has been successfully employed for editing genomes of various organisms. Here we describe a protocol for editing the vaccinia virus (VV) genome in the cytoplasm of V...
Gespeichert in:
| Veröffentlicht in: | J Vis Exp |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
MyJove Corporation
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5226142/ https://ncbi.nlm.nih.gov/pubmed/27842362 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54171 |
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