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A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9
The current method for creation of vaccinia virus (VACV) vectors involves using a selection and purification marker, however inclusion of a gene without therapeutic value in the resulting vector is not desirable for clinical use. The Cre-LoxP system has been used to make marker-free Poxviruses, but...
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| Pubblicato in: | Mol Ther Methods Clin Dev |
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| Autori principali: | , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4571730/ https://ncbi.nlm.nih.gov/pubmed/26417609 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtm.2015.35 |
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