TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus

Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) s...

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發表在:Nucleic Acids Res
Main Authors: Senís, Elena, Mockenhaupt, Stefan, Rupp, Daniel, Bauer, Tobias, Paramasivam, Nagarajan, Knapp, Bettina, Gronych, Jan, Grosse, Stefanie, Windisch, Marc P., Schmidt, Florian, Theis, Fabian J., Eils, Roland, Lichter, Peter, Schlesner, Matthias, Bartenschlager, Ralf, Grimm, Dirk
格式: Artigo
語言:Inglês
出版: Oxford University Press 2017
主題:
Methods Online
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5224498/
https://ncbi.nlm.nih.gov/pubmed/27614072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw805
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