TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus

Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) s...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Senís, Elena, Mockenhaupt, Stefan, Rupp, Daniel, Bauer, Tobias, Paramasivam, Nagarajan, Knapp, Bettina, Gronych, Jan, Grosse, Stefanie, Windisch, Marc P., Schmidt, Florian, Theis, Fabian J., Eils, Roland, Lichter, Peter, Schlesner, Matthias, Bartenschlager, Ralf, Grimm, Dirk
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5224498/
https://ncbi.nlm.nih.gov/pubmed/27614072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw805
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados