TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus

Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) s...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Senís, Elena, Mockenhaupt, Stefan, Rupp, Daniel, Bauer, Tobias, Paramasivam, Nagarajan, Knapp, Bettina, Gronych, Jan, Grosse, Stefanie, Windisch, Marc P., Schmidt, Florian, Theis, Fabian J., Eils, Roland, Lichter, Peter, Schlesner, Matthias, Bartenschlager, Ralf, Grimm, Dirk
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2017
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5224498/
https://ncbi.nlm.nih.gov/pubmed/27614072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw805
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!

Lignende værker