Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limitations by developing single-step optimized inducibl...

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Dades bibliogràfiques
Publicat a:Development
Autors principals: Bertero, Alessandro, Pawlowski, Matthias, Ortmann, Daniel, Snijders, Kirsten, Yiangou, Loukia, Cardoso de Brito, Miguel, Brown, Stephanie, Bernard, William G., Cooper, James D., Giacomelli, Elisa, Gambardella, Laure, Hannan, Nicholas R. F., Iyer, Dharini, Sampaziotis, Fotios, Serrano, Felipe, Zonneveld, Mariëlle C. F., Sinha, Sanjay, Kotter, Mark, Vallier, Ludovic
Format: Artigo
Idioma:Inglês
Publicat: The Company of Biologists Ltd 2016
Matèries:
Stem Cells and Regeneration
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5201041/
https://ncbi.nlm.nih.gov/pubmed/27899508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.138081
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