Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limitations by developing single-step optimized inducibl...

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Библиографические подробности
Опубликовано в: :Development
Главные авторы: Bertero, Alessandro, Pawlowski, Matthias, Ortmann, Daniel, Snijders, Kirsten, Yiangou, Loukia, Cardoso de Brito, Miguel, Brown, Stephanie, Bernard, William G., Cooper, James D., Giacomelli, Elisa, Gambardella, Laure, Hannan, Nicholas R. F., Iyer, Dharini, Sampaziotis, Fotios, Serrano, Felipe, Zonneveld, Mariëlle C. F., Sinha, Sanjay, Kotter, Mark, Vallier, Ludovic
Формат: Artigo
Язык:Inglês
Опубликовано: The Company of Biologists Ltd 2016
Предметы:
Stem Cells and Regeneration
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5201041/
https://ncbi.nlm.nih.gov/pubmed/27899508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.138081
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