Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limitations by developing single-step optimized inducibl...

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Detalles Bibliográficos
Publicado en:Development
Autores principales: Bertero, Alessandro, Pawlowski, Matthias, Ortmann, Daniel, Snijders, Kirsten, Yiangou, Loukia, Cardoso de Brito, Miguel, Brown, Stephanie, Bernard, William G., Cooper, James D., Giacomelli, Elisa, Gambardella, Laure, Hannan, Nicholas R. F., Iyer, Dharini, Sampaziotis, Fotios, Serrano, Felipe, Zonneveld, Mariëlle C. F., Sinha, Sanjay, Kotter, Mark, Vallier, Ludovic
Formato: Artigo
Lenguaje:Inglês
Publicado: The Company of Biologists Ltd 2016
Materias:
Stem Cells and Regeneration
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5201041/
https://ncbi.nlm.nih.gov/pubmed/27899508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.138081
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