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A novel strategy for the identification of protein–DNA contacts by photocrosslinking and mass spectrometry
Photochemical crosslinking is a method for studying the molecular details of protein–nucleic acid interactions. In this study, we describe a novel strategy to localize crosslinked amino acid residues that combines laser-induced photocrosslinking, proteolytic digestion, Fe(3+)-IMAC (immobilized metal...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2004
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC519130/ https://ncbi.nlm.nih.gov/pubmed/15383647 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh131 |
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