DNA sequencing and genotyping by transcriptional synthesis of chain-terminated RNA ladders and MALDI-TOF mass spectrometry

Sets of RNA ladders can be synthesized by transcription of a bacteriophage-encoded RNA polymerase using 3′-deoxynucleotides as chain terminators. These ladders can be used for sequencing of DNA. Using a nicked form of phage SP6 RNA polymerase in this study substantially enhanced yields of transcript...

Deskribapen osoa

Xehetasun bibliografikoak

Argitaratua izan da:	Nucleic Acids Res
Egile Nagusiak:	Kwon, Young-Soo, Tang, Kai, Cantor, Charles R., Köster, Hubert, Kang, Changwon
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	Oxford University Press 2001
Gaiak:	NAR Methods Online
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC30412/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11160913/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.3.e11
Etiketak:	Etiketa erantsi Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!