DNA sequencing and genotyping by transcriptional synthesis of chain-terminated RNA ladders and MALDI-TOF mass spectrometry

Sets of RNA ladders can be synthesized by transcription of a bacteriophage-encoded RNA polymerase using 3′-deoxynucleotides as chain terminators. These ladders can be used for sequencing of DNA. Using a nicked form of phage SP6 RNA polymerase in this study substantially enhanced yields of transcript...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Kwon, Young-Soo, Tang, Kai, Cantor, Charles R., Köster, Hubert, Kang, Changwon
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2001
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC30412/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11160913/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.3.e11
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