DNA sequencing and genotyping by transcriptional synthesis of chain-terminated RNA ladders and MALDI-TOF mass spectrometry

Sets of RNA ladders can be synthesized by transcription of a bacteriophage-encoded RNA polymerase using 3′-deoxynucleotides as chain terminators. These ladders can be used for sequencing of DNA. Using a nicked form of phage SP6 RNA polymerase in this study substantially enhanced yields of transcript...

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Hlavní autoři: Kwon, Young-Soo, Tang, Kai, Cantor, Charles R., Köster, Hubert, Kang, Changwon
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2001
Témata:
NAR Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC30412/
https://ncbi.nlm.nih.gov/pubmed/11160913
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