A novel strategy for the identification of protein–DNA contacts by photocrosslinking and mass spectrometry
Photochemical crosslinking is a method for studying the molecular details of protein–nucleic acid interactions. In this study, we describe a novel strategy to localize crosslinked amino acid residues that combines laser-induced photocrosslinking, proteolytic digestion, Fe(3+)-IMAC (immobilized metal...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2004
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC519130/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15383647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh131 |
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