A novel strategy for the identification of protein–DNA contacts by photocrosslinking and mass spectrometry

Photochemical crosslinking is a method for studying the molecular details of protein–nucleic acid interactions. In this study, we describe a novel strategy to localize crosslinked amino acid residues that combines laser-induced photocrosslinking, proteolytic digestion, Fe(3+)-IMAC (immobilized metal...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Geyer, Hildegard, Geyer, Rudolf, Pingoud, Vera
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2004
Assuntos:	NAR Methods Online
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC519130/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15383647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh131
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