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Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts

The construction of knockin vectors designed to modify endogenous genes in embryonic stem (ES) cells and the generation of mice from these modified cells is time consuming. The timeline of an experiment from the conception of an idea to the availability of mature mice is at least 9 months. We descri...

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Bibliografiske detaljer
Main Authors: Zhou, Dewang, Ren, Jin-Xiang, Ryan, Thomas M., Higgins, N. Patrick, Townes, Tim M.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC519128/
https://ncbi.nlm.nih.gov/pubmed/15356288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh128
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