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Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts

The construction of knockin vectors designed to modify endogenous genes in embryonic stem (ES) cells and the generation of mice from these modified cells is time consuming. The timeline of an experiment from the conception of an idea to the availability of mature mice is at least 9 months. We descri...

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Autores principales: Zhou, Dewang, Ren, Jin-Xiang, Ryan, Thomas M., Higgins, N. Patrick, Townes, Tim M.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2004
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC519128/
https://ncbi.nlm.nih.gov/pubmed/15356288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh128
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