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Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts

The construction of knockin vectors designed to modify endogenous genes in embryonic stem (ES) cells and the generation of mice from these modified cells is time consuming. The timeline of an experiment from the conception of an idea to the availability of mature mice is at least 9 months. We descri...

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Main Authors: Zhou, Dewang, Ren, Jin-Xiang, Ryan, Thomas M., Higgins, N. Patrick, Townes, Tim M.
格式: Artigo
語言:Inglês
出版: Oxford University Press 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC519128/
https://ncbi.nlm.nih.gov/pubmed/15356288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh128
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