Guide RNA engineering for versatile Cas9 functionality

The Clustered Regularly Interspaced Short Palindromic Repeats system allows a single guide RNA (sgRNA) to direct a protein with combined helicase and nuclease activity to the DNA. Streptococcus pyogenes Cas9 (SpCas9), a CRISPR-associated protein, has revolutionized our ability to probe and edit the...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Nowak, Chance M., Lawson, Seth, Zerez, Megan, Bleris, Leonidas
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2016
Onderwerpen:
Survey and Summary
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5175371/
https://ncbi.nlm.nih.gov/pubmed/27733506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw908
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