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Guide RNA engineering for versatile Cas9 functionality
The Clustered Regularly Interspaced Short Palindromic Repeats system allows a single guide RNA (sgRNA) to direct a protein with combined helicase and nuclease activity to the DNA. Streptococcus pyogenes Cas9 (SpCas9), a CRISPR-associated protein, has revolutionized our ability to probe and edit the...
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| Udgivet i: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5175371/ https://ncbi.nlm.nih.gov/pubmed/27733506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw908 |
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