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Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

BACKGROUND: Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucia...

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Библиографические подробности
Опубликовано в: :Microb Cell Fact
Главные авторы: Jahn, Michael, Vorpahl, Carsten, Hübschmann, Thomas, Harms, Hauke, Müller, Susann
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5168713/
https://ncbi.nlm.nih.gov/pubmed/27993152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0610-8
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