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Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

BACKGROUND: Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucia...

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Publicado en:Microb Cell Fact
Main Authors: Jahn, Michael, Vorpahl, Carsten, Hübschmann, Thomas, Harms, Hauke, Müller, Susann
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5168713/
https://ncbi.nlm.nih.gov/pubmed/27993152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0610-8
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