Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave

Fluorescence techniques for monitoring single-molecule dynamics in the vertical dimension currently do not exist. Here we use an atomic force microscope to calibrate the distance-dependent intensity decay of an evanescent wave. The measured evanescent wave transfer function was then used to convert...

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Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Sarkar, Atom, Robertson, Ragan B., Fernandez, Julio M.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2004
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC516489/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15326308/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0403534101
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