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Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave
Fluorescence techniques for monitoring single-molecule dynamics in the vertical dimension currently do not exist. Here we use an atomic force microscope to calibrate the distance-dependent intensity decay of an evanescent wave. The measured evanescent wave transfer function was then used to convert...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC516489/ https://ncbi.nlm.nih.gov/pubmed/15326308 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0403534101 |
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