Exon amplification: a strategy to isolate mammalian genes based on RNA splicing.

We have developed a method, exon amplification, for fast and efficient isolation of coding sequences from complex mammalian genomic DNA. This method is based on the selection of RNA sequences, exons, which are flanked by functional 5' and 3' splice sites. Fragments of cloned genomic DNA are inserted...

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發表在:	Proc Natl Acad Sci U S A
Principais autores:	Buckler, A J, Chang, D D, Graw, S L, Brook, J D, Haber, D A, Sharp, P A, Housman, D E
格式:	Artigo
語言:	Inglês
出版:	National Academy of Sciences 1991
主題:	Research Article
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51582/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1850845/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.9.4005
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